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The constraints of vanilla development in conventional vegetative propagation are the presence of Fusarium wilt disease and the limitations of the parent plant as cuttings so that vanilla is developed through micro propagation techniques. The ability of the explants to regenerate and differentiate to form buds in vitro is excessive need to be controlled through cytokine regulation in an effort to get the same vanilla seedlings as parent, healthy and uniform. The objectives of this research are: 1) developing vanilla seedlings through multiplication of shoots by modifying BAP and Kinetin in vitro and 2) obtaining vanilla seeds through uniform, healthy and not attacked by plant disease pests through micro propagation techniques. The study was conducted in the Jember State Polytechnic Network Culture laboratory using a factorial Complete Randomized Design (CRD). Factor 1 is BAP concentration that is 0, 1, 2, 3, 4 ppm and factor 2 is Kinetin concentration which is 0, 1, 2 ppm. Further data analysis used Duncans average difference test of 5% or 1%. The parameters observed in this study were: 1) the budding speed, 2) the number of shoots, 3) the length of shoots, 4) the wet weight of shoots and 5) the development of explants. Achievement of the expected results in this study has a value of Technological Adequacy Level (TKT) 5 which is a picture of validation of cytokine modifications that effectively produce multiplication of vanilla shoots so that the same vanilla seedlings are obtained as the parent, uniform, healthy.