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The aims of this study to determine the process of development, the fesiability and students reponse to the material instructional based on local wisdom in Bali for first grade of senior high school. This material instructional uses the ADDIE development model which consists of five steps. ADDIE steps include analysis, design, development, implementation, and evaluation. The development instructional materials based on suitable materials component, suitable presentation, suitable language, and suitable graphics from Badan Standar Nasional Pendidikan (BSNP). The techniques of data collection use questionnaire of validation sheet and students response sheet. Data analysis using qualitative descriptive. An expert review of the results of the assessment shows a value of 88,17% (valid) for expert material and 90,08% (valid) for media experts. Meanwhile the result of students response is 90,91% which categories is very strong. Based on validity results by experts and students reponse, then the instructional materials based on Bali local wisdom in environmental conservation can be used for biology learning. Further research is recommended to look at the impact of this instructional materials on 21st century skills of high school students.