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Exercise is a form of physical exercise that can improve physical fitness if done with the right frequency and intensity. Regular and measured exercise requires various training programs, such as interval training, circuit training, continuous training, combination exercise, or weight exercise, each of which has measurable exercise ratio to individual needs. Regular and measured exercise will result in increased work of the respiratory system, cardiovascular system, strengthen skeletal muscles and endurance. The purpose of this research is to analyze the effect of exercise ratio 1:2 on changes of anaerobic threshold and lactic acid levels in students of Physical Education, Health, and Recreation Study Program, Sport Education Department, Faculty of Sport Sciences, Universitas Negeri Yogyakarta (PJKR FIK UNY). The research method was by quasi-experimental. Research design used pretest-posttest group design, with quantitative research type. Data collection for the variables studied was done by test instrument (1) anaerobic threshold test using Fitmate version 2.2, Wellness Technology a Division of Cosmed and treadmill, (2) lactic acid level test using Accutrend Plus. Research results indicate that there is significant effect between exercise ratio 1:2 on anaerobic threshold and lactic acid level changes of PJKR FIK UNY students