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Purpose: The purpose of this study is to investigate and analyze the expression of FBP1 and LDH-B enzymes in benign and malignant tumors Method: This study using an experimental method with a cross-sectional approach. A total of 63 samples of both benign and malignant tumor tissue in the breast where testing was performed using immunohistochemical techniques to calculate the percentage and intensity of expression of both the FBP1 enzyme and the LDH-B enzyme. Following international standards, the expression of the FBP1 and LDH-B enzymes is positive if the immunoreaction detected in the cytoplasm is ≥ 4. Results: This study shows that the FBP1 enzyme is expressed in both benign tumor samples (68.7%) and malignant tumors (71 %) with the Chi-Square test results showing a p-value of 1,000 which means there is no significant difference from the expression of the FBP1 enzyme that is associated with tumor status. LDH-B enzymes were also expressed both in benign tumor samples (91%) and in malignant tumors (64.5%) but their expression decreased in malignant tumors with Chi-Square test results showed a p-value of 0.029 which means there was a significant difference in expression LDH-B enzymes are associated with tumor status. Conclusion: This study shows that the FBP1 and LDH-B enzymes are expressed by both benign and malignant tumor tissue, but there is a significant difference between LDH-B enzyme expression and tumor status as indicated by a significant decrease in LDH-B enzyme expression in malignant tumor tissue breast which is a possible marker of changes in cell metabolism related to the presence and function of lactate in the metabolism of malignant breast tumor cells.