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Background: A Diphtheria Pertussis Tetanus (DPT) immunization often causes trauma in infants because it is done by intramuscular injection. Purpose: The aim of this study is to determine the effect of facilitated tucking by mother combined with sitting up position on the level of pain when infants receive DPT immunization. Method: A randomized controlled trial design with post-test equivalent group technique was used. Three groups (intervention by mothers, intervention by health worker, and control group) were randomly selected, with each consisting of 16 infants. Results: Data were analyzed using the Kruskal Wallis test with a ρ value=0.006 (< 0.05). The result of the analysis conducted by post hoc Mann-Whitney test revealed that (1) intervention by mother vs intervention by health worker group have a ρ value=0,039 (<0.05); (2) intervention by mother vs control group have a ρ value=0,002; and (3) intervention by health workers vs control group have a ρ value=0,195. Conclusion: There was a significant difference in the level of infant pain between intervention by mother vs intervention by health worker, and intervention by mother vs control group. Recommendation: It is recommended that facilitated tucking by mother combined with sitting up position should be used in infant pain management during DPT immunization.