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Objective: This study aims to determine the effectiveness of Centella asiatica (L). Urban extraction TNF-α levels in female Sprague dawley rats induced by S. aureus. Methods: There were four treatment groups (each group consisted of five rats). Before treatment, S. aureus 0.2 mlx108 CFU was induced. Group I was only given 0.5% Na CMC, Cefadroxil group II 45 mg / kg WB, Group III Centella leaf extract 100 mg / Kg WB, and group IV combination Cefadroxil and Centella leaf extract. Each group was given treatment twice per 12 hours a day for 5 days. TNF-α levels were measured on day 3 and day 6 after induction of S. aureus tested by ELISA and analyzed by One Way ANOVA test and independent sample T test. Results: TNF-α level between groups did not have a significant on day 3 and differed significantly after day 6. On day 3, the control group had a higher TNF-α level of 25.13 pg/ml compared to the group given antibiotics and Centella asiatica. While when compared to the group given only Centella asiática the control group was more height of 17.1 pg /ml. On the 6th day this condition was changed, the biggest difference found in the group given Centella asiatica, in the control group had higher levels of TNF-α 72.34 pg / ml compared to the group receiving Centella asiática. Then, control group is higher than 66.46 pg/ml compared to those given antibiotics and Centella asiatica. Conclusion: C. asiatica leaf extract is effective in reducing TNF both given alone and given along with antibiotics. It is potential to be explored into alternative and complementary treatments in mastitis cases with human trials.