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Brucellosis in cattle is a zoonotic disease that causes abortion. This disease is highly contagious and difficult to control. Clinical symptoms Brucellosis is not pathognomonic and often asymptomatic thereof laboratory assessment is needed for serological detection of Brucellosis. A total of 235 blood samples were obtained from Bali cattle farms in three regencies, Enrekang, Barru, and Soppeng. Serological detection was carried out by the RBT method and further confirmed by CFT method. Results showed that Brucellosis seroprevalence was 12.76%. The highest incidence rate was in Enrekang Regency with the value of 15.60%, followed by Soppeng and Barru Regency, of 9.09% and 2.50%, respectively. These results indicated that Brucellosis is still enzootic in the South Sulawesi region.