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Background: Java Barb (Puntius javanicus) is one of the Indonesian native fish which is easily cultivated and has good demand with high price (Budiharjo, 2001). There were fish farmers in Muntilan, Central Java who have practiced very intensive aquaculture. One of the problems faced by the fish farmers from the practices was a decrease in water quality that slowed down the fish growth and also increased fish diseases that brought down fish production. One of the solutions to solve the problems was by adding immunostimulant yeast (Saccharomyces cerevisiae) into feed to promote growth and to improve immune system in the fish in order to protect from the diseases. The study was conducted at the Laboratory for Fish Health and Environment Assessment (Laboratorium Pengujian Kesehatan Ikan dan Lingkungan -LPKIL), Muntilan, Jawa Tengah, Indonesia on April to July 2019. According to [1] immunostimulant yeast (S. cerevisiae) supplemented feed can improve growth rate and fish health. Moreover, [2] reported that β-glucan content in the yeast can boost immune system and disease resistance in the fish. Aims: The study aims to evaluate effects of immunostimulant yeast (Saccharomyces cerevisiae) supplemented feed on growth rate and blood profile in Java Barb (P. javanicus). Materials and Methods: The 200 fingerlings of Java barb (P. javanicus) used in the study had an average weight of 5.64±0.87 g/fish. The study implemented experimental method using Completely Randomized Design with 5 (five) treatments and 4 (four) replicates. The treatments were by adding various doses of immunostimulant yeast (S. cerevisiae) into feed. Those treatment were T1 (0 g /kg feed), T2 (1 g /kg feed), T3 (2 g/kg feed), T4 (3 g/kg feed), and T5 (4 g/kg feed). The observed variables were immune system, Efficiency of Feed utilization (EFU), Feed Conversion Ratio (FCR), Protein Efficiency Ratio (PER), Specific Growth Rate (RGR), Survival Rate (SR), blood profile and water quality. Statistical analysis using an Analysis of Variance (ANOVA) and Duncan test was employed to analyze observed variables [3]. The measurement of optimal dose of immunostimulant yeast (S. cerevisiae) in the feed used polynomial orthogonal test with SAS9 and Maple12. Water quality was descriptively analyzed by comparing to the references to determine the water viability. Results: The results showed that the supplementation of immunostimulant yeast (S. cerevisiae) in the feed significantly (P<0.05) influenced on EFU, FCR, PER and RGR; otherwise, it did not significantly influence on SR of Java Barb. The supplementation of immunostimulant yeast (S. cerevisiae) into the feed was expected to increase EFU, PER, RGR and to decrease FCR in Java barb. According to [4] that supplementation of immunostimulant yeast (S. cerevisiae) into the feed could increase enzyme activities in the digestive system. In turn, it could break down complex compound of the nutrients into simpler form of the nutrients