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Experiences or negative attitudes towards HIV, low knowledge and perception are also factor that can influence the emergence of stigma and discrimination. This study was designed with the primary objective of assessing the knowledge, attitude and perception among Faculty of Health Science students of Universitas Muhammadiyah Magelang towards risk of HIV/AIDS. The data was collected by questionnaire from students in five department Faculty of Health Science (n= 165, response rate 82.5%). Each respondent-s scores were totaled and individual average scale scores were analyzed using descriptive analysis. Overall, the level of knowledge Faculty of Health Sciences students towards risk of HIV and AIDS was moderate (range 51-74) of 53.34%. The attitude of health science faculty students is quite positive. The average scale score for attitudes towards risk of HIV and AIDS is 0.63 (min = 0, max = 1) of 62.4%. The perception of health faculty students is quite negative. The average scale score for perception of HIV and AIDS risk is 0.63 (min = 0, max = 1) of 51.5%. Increase knowledge about HIV/AIDS through education and training since attending college, providing sufficient and up-to-date information about HIV/AIDS so as to be able to prepare health science faculty students to treat patients with HIV/AIDS and reduce negative stigma and discrimination.