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The presence of spermatozoa in the vagina is a sure sign of intercourse. In some cases microscopic examination give no evidence or establish false negative results. This may either be by absence of ejaculate, oligo/azoospermia, vasectomy, sperm degeneration or sampling and storage factors. Under such condition, other ejaculate components, namely the enzyme acid phosphatase, choline has to be thought. To establish the effectiveness of such ejaculate components/test, this study through direct and indirect method examined 192 clothes semen patches by phosphatase acid and zinc test on day 1, 7 and 3 months. On the first method, washing was done then examined; whereas the second method had simultaneous washing and tests with 7 different brands of detergents on 4 different semen patches and water as a control. The results showed that the sensitivity was very low (0.186) while the specificity was very high (100%). This shows that both tests have high specificity values: phosphatase test for the presence of phosphatase enzyme, zinc test for zinc in semen. Despite of the specificity, phosphohydrolase-phosphatase enzyme is though susceptible to degradation by temperature, humidity and chemical substance of element SDS (Sodium Dodecyl Sulfate) which has the ability to cut enzymes.