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Abstract: Objectives: Mastitis, a breast inflammation caused by Staphylococcus aureus infection can increase mediator level of TNF-α. Pro-inflammation. The research aims to analyze the effect of beruwas laut leaf extract (Scaevola taccada (Gaertn.) Roxb.) on TNF-α levels. Methods: Posttest control group design was applied in this research. Sample consisted of 18 female rats (Sprague Dawley) which were divided into 3 groups, such as negative control, positive control, and 400 mg/kg body weight extract doses. TNF-α level was examined using ELISA method. The data were analyzed using paired t-test and Kruskal Wallis. Results: Our results indicate that there was no significant difference between TNF-α level after induction of bacteria and the one after giving treatment (p = 0.973) in negative control group. While in positive control group, significant difference was found between TNF-α after induction of bacteria and the one after giving antibiotic (p = 0.026). Significant difference was also found in 400 mg/kg birth weight extract doses group (p = 0.038). After the induction of bacteria, it is indicated that there was no difference of TNF - α level among the three groups (p = 0.579). After giving treatment, there was a difference among the three groups (p = 0.017). Conclusion: Extract doses of 400 mg/kg birth weight cannot compete the effect of antibiotic amoxicillin in reducing TNF-α level. However, both of them have similar effect. Therefore, beruwas leaf extract can be used as a complementary therapy (supplement) in mastitis treatment