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Compost is one of the processes of decomposing organic materials that involves the activity of microorganisms partially. The use of decomposers is very important to accelerate the rate of compost fermentation. The study was aims to evaluate the quality of compost produced from different types of decomposer substrates and straw compositions. This study uses feces from Bali cattle as main material which are maintained in a semi-intensive manner. Two types of decomposer substrate were used, namely (1) animal substrate and (2) vegetable substrate. The level of straw applied were (1) 0%, (2) 5% and (3) 10% (from the total of compost). The study was conducted based on a completely randomized design (CRD) factorial pattern. The data were analyzed using ANOVA. The results showed that differences in the type of decomposer substrate and straw composition had no significant effect (P>0.05) on the pH value, levels of C-organic, N-organic and C/N ratio of compost. The pH value is in the range of 7.4-7.69. The value of C-organic content varies with a range of values 15.29-18.52%. N-organic content is in the range of 0.72 to 0.97% and C / N ratio of 19.00-25.66. The final results of the study showed that the use of animal substrate decomposer and using 10% straw from the from the total of compost showed better compost quality compared to other treatments.