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In the development of new drugs as candidates for cancer therapy agents, cytotoxictesting is needed as an initial screening to determine the effect of a natural substance ininhibiting tumor cell growth. One of the active compounds of flavonoid derivatives that are being studied as anticancer is a compound derived from Gynura divaricata extract. The purpose of thisstudy was to determine the cytotoxic activity of flavonoid compounds from Gynura divaricata water extract on carcinoma mammae cell culture (T47D) and make comparisons with standard drugs for cancertherapy. Examination of cytotoxic activity was carried out on T47D cell line culture used MTT method. The absorbance of each well was measured with spectrophotometer at a wave length of 595 nm. The absorbance results was calculated to create a cytotoxic curve used Probhit analysis. Based on the cytotoxic curve, IC50 values of Gynura divaricata extract and doxorubicin were 102,32μg/mL and 10,30μg/mL respectively. Those showed thatGynura divaricata water extract have anti-cancer activity (IC50<200μg/mL)against carcinoma mammae cells culture (T47D) compare to doxorubicin. It was concluded that Gynura divaricata water extract were shown to have anticancer activity on carcinoma mammae cells culture (T47D), but not as strong as doxorubicin .