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The use of non-steroidal anti-inflammatory drugs (NSAIDs) in chronic pain in the long term can cause side effects especially disorders of the digestive tract. In Usada Bali, one of the plants used to reduce pain is the Liligundi (Vitex trifolia L.) family of Lamiaceae. This study was aimed to prove the analgesic activity of Liligundi leaves extract in mice. Liligundi leaves was extracted using 70% alcohol solvent followed by phytochemical screening and analgesic activity test using hot plate method with temperature of 55oC in 20 mice which were divided into four groups. Negative control group was given Tween 80, positive control group was given Ibuprofen 52 mg/kg BW, and treatment of Liligundi Leaves Extract (LLE) dose of 250 mg/kgBW and 500 mg/kgBW. Pain reaction in mice was measured 30th minutes after treatment, marked by jumping or licking the feet after being placed on a hot plate. Data were analyzed by ANOVA and Tukey test. From the study we find that LLE contains alkaloids, flavonoids, saponins, tannins and terpenoids. LLE doses of 250 mg/kgBW and 500 mg/kgBW significantly extend the pain response compared to negative controls and produce a significantly longer pain response reaction time than Ibuprofen (p<0.05).