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Series of ferrogel samples have been successfully fabricated by using the freezing-thawing role. The filler used in ferrogel is magnetic material of Mn0.25Fe2.75O4/Ag which is synthesized by the co-precipitation method and chemical reduction. Whereas, the matrix used in ferrogel is a polymer composite of Carboxymethyl Cellulose (CMC) and Polyvinyl Alcohol (PVA). The characterization of Mn0.25Fe2.75O4/Ag powder was carried out by using X-Ray Diffraction (XRD), Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM), and antibacterial activities. While the Mn0.25Fe2.75O4/Ag-CMC / PVA ferrogel was successfully characterized by antibacterial activity using the agar diffusion method. The XRD pattern of the powder of Mn0.25Fe2.75O4/Ag illustrates the peaks owned by Fe3O4 and silver phases. The morphology of the Mn0.25Fe2.75O4/Ag powder using TEM characterization shows the particles size of the Fe3O4 and Ag is 5.03 and 4.34 nm respectively. Meanwhile, the magnetization hysteresis curve of Mn0.25Fe2.75O4/Ag showed a decrease from 20.74 emu / g to 15.53 emu / g along with the addition of Ag material. Through the agar diffusion method, samples of Mn0.25Fe2.75O4/Ag powder and Mn0.25Fe2.75O4/Ag -CMC/PVA ferrogel showed excellent antibacterial activity as indicated by the widening of zones that were not overgrown with bacteria around the sample. Thus, the Mn0.25Fe2.75O4/Ag powder and Mn0.25Fe2.75O4/Ag-CMC/PVA ferrogel is potential for use in the biomedical field.