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Handline tuna fisheries in Palabuhanratu have developed since 2004 and categorized as small-scale fisheries. Economic sustainability is one of the objectives of the handline tuna operation. This paper aims to analyze the cost and revenues of tuna fishing resources by using a handline in Palabuhanratu. The results indicate the handline catch rate has increased from the years 2012-2014 with an average 657kg / trip. The investment cost of handline tuna fishing operation is $19,792.86,-, with depreciation charge of $5,525.00,-/year, maintenance cost amount $2,589.29,-/year and operational cost of $16,222,-/year. The revenue from the fishing business is $44,057,-/year. The business profit amount USD $ 301,-/trip or USD $ 7,217,-/year. Based on investment analysis the value of R / C = 1,32, PP = 1,86, NPV = $ 24,528.27,-, B/C = 5,27 and IRR = 119%, where it shows that fishing is profitable, with a relatively quick payback and sustainable.