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This study aims to determine the blue laser diode irradiation angle and the optimum time for bacterial photoinactivation. Staphylococcus aureus. The research was carried out at various angles and lengths of irradiation time. Data analysis using anova factorial to determine the effect of each factor. The results showed the influence of blue diode laser irradiation angle on inactivation of Staphylococcus aureus bacteria. Laser diode 403 nm exposure with an angle of 90 lurus perpendicular to the surface of the absorbent medium and exposure time (90 ± 0.005) is optimal for reducing bacterial viability with a percentage decrease in bacterial viability of (72.7814 ± 4.1361)% not significantly different from the 80o angle. So the angle of exposure and duration of irradiation has an effect on the photodynamic effectiveness of bacterial inactivation.