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The aim of this research was to determine the effect of the level of phosphorus fertilizer on the growth and dry matter production Stylosanthes guianensis. This research aim know the effect of phosphorus fertilizer application on growth and dry matter production of stylo (Stylosanthes guianensis). This research is based on Completely Randomized Design (CRD) with 4 treatments and 4 replicates in order to obtain 16 experimental units. The treatments of this research are P0 (Control) P1 (0.75 g. TSP / polybag), P2 (1.5 g. TSP / polybag), P3 (2.25 g. TSP / polybag). The Parameters are plant height, number of leaf branches, number of branches plant, dry matter production, and the number of nodules. The results showed that the treatments has not significantly affected (P> 0.05) plant height, number of stems, number of branches, and dry matter production, but it has very significant effect (P <0.01) on the number of nodules. However, P3 Treatment is better to use than the other treatments.