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Zinc oxide and eugenol (ZOE) dental cements are widely used in dental restorations. This cement has low heat transfer properties, protects the pulp from phosphoric acid of phosphate cement or silicates and does not irritate the pulp. But ZOE has lowest mechanical properties and high solubility properties. From these shortcomings, it is necessary to change the size of zinc oxide material. The size of the micrometer is changed to nanoparticles in order to accelerate the formation of cement and increase the strength of cement. The sample used in this in vivo study was made from zinc oxide and eugenol dental cement and zinc oxide nanoparticles. Nano cement of this tooth has a composition of zinc oxide of 0.4 g, 0.45 g, 0.5 g and 0.55 g and eugenol 0.2 ml liquid. The experimental animals used in this study were Riverine rabbit species (Bunolagus monticularis). The interaction time of nano zinc oxide dental cement in rabbit teeth are 7, 14, 21 and 30 days. Based on the results of compressive strength test, it is obtained that the compressive value is higher in the composition of nano zinc oxide eugenol 0.55 g. Furthermore, along with the longer duration of interaction time between nano zinc oxide eugenol dental cement in rabbit teeth the better result obtained. This is supported by the microstructure of the sample captured using Scanning Electron Microscopy (SEM). SEM result is indicated disguised connection between the zinc oxide eugenol dental cement and rabbit teeth.