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This study has been done to know the effectiveness of ecology and environment textbook based on science literacy and local potency of north sumatra in improving student science literacy in biology learning process on senior high school. The effectiveness of the text book was based on students learning outcome in aspect of biology as a body of knowledge, biology as a way of investigation, biology as way of thinking and interaction of science, technology and society. The effectiveness testing of the text book was carried out in two senior high schools, namely in Markus senior high school and Free Methodist senior high school.This research was quasi experiment with two classes served as sample which taken by cluster random sampling. The first class (X1) was taught by using conventional biology handbook and the second class (X2) was taught by using ecology and environment textbook that has been developed namely text book based on science literacy and north sumatras local potency. The science literacy of the student was measured by using science literacy test which consisted of essay test, multiple choices test and observation sheet. Data analysis technique was done by using student t test with helping SPSS 22.0 and also by ngain score. The research result showed that n-gain score in the class that using ecology and environment text book was higher (ngain score=0.65) than in the class that using conventional text book (ngain score = 0.4). This result indicated, the text book that has been developed was effective in the improving the science literacy of the students. Student t test result furthermore showed that there was a very significantly differences in student science literacy (tcount=10.45; P=0.001) between class that taught by using ecology and environment textbook based on science literacy and north sumatras local potency ( SD = 79.86 4.21) and the class that be taught by using conventional biology text book ( SD = 68.01 5.71). This result confirmed that textbook based on science literacy and North Sumatras local potency was more effective in increasing the science literacy of the students. This research result recommend the using of ecology and environment text book based on science literacy and north sumatera local potency in the improving of student science literay in biology learning process in senior high school in north sumatera province Indonesia.