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The tubers plant of the Dioscorea family is a carbohydrate-producing plant suitable for growing in Indonesia and has the potential to be developed to support diversification of food and energy which is increasingly important due to the reduced fossil energy. The Yam (Dioscorea alata L.) are easy to growth, and has a broad adaptability in terms of altitude and astronomical boundaries. In Indonesia, where the main food ingredients are rice, corn and cassava, making the yam potencial plant an alternative carbohydrate producer that will not compete with rice and cassava to support alternative energy. The research aims to obtain optimal cultivation techniques through efficient use of seeds and fertilizing, to provide sufficient carbohydrates for bioethanol raw materials from yam. The research was carried out in the Station Research of the Faculty of Agriculture, Merdeka Madiun University, done in the rainy season of November 2018 until July 2019. Tuber seed size and Fertilizer combination be tested by RCBD and analysis by F-test and DMRT. No interaction between fertilizer combination and size of tuber seed. Combination of organic fertilizer and addition of NPK inorganic fertilizers showed a significant response to the increase in tuber weight and biomass dry matter. While the size of seed tubers, the difference in size / weight does not significantly affect the tuber yield, although there is a tendency to increase with increasing tuber weight