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Synthetic dyes can cause health and environmental impacts, thus, there are opportunities to develop natural dyes, one of which is produced by Indigofera tinctoria plants. This plant is from fabaceae that has the potential to produce a natural blue color. Natural dyes are extracted from the leaves of plants that contain indigo compounds. Indigo growth and precursors are very dependent on environmental conditions, one of which is light intensity. This study aimed to study the morphological and physiological plant responses in Indigofera tinctoria to several levels of light intensity. The research was conducted in Puron Village, Sukoharjo with a complete randomized block design (RCBD) one factor namely the level of light intensity (100%, 50% and 25%) with 9 replications. Light intensity affected the morphology and physiology of Indigofera tinctoria. Plants responded to low light intensity by increasing the leaf area index, specific leaf area and plant height. Leaf area, specific leaf area and plant height were highest at 25% intensity. However, the number of leaves and nodes got greater at full light intensity. Higher light intensity increased the chlorophyll content a, b and total, thus, higher biomass yield which was 18.86 g at the age of 8 weeks.