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Background : Pneumonia Associated Ventilator (VAP) is the most common pneumonia in the Intensive Care Unit (ICU) and is one of the causes of the highest mortality of up to 67%. This research objective is to know the effect of combination in using natrium bicarbonate and listerine® to oral hygiene toward incidence VAP in Pusat Otak Nasional Hospital. Methodology : Quantitative reseach with quasi experiment (pretest-posttest with control group design) program. The sample is taken using purposive sampling technique as many as 30 respondents. This research instrument using CPIS observation, data analysis using Willcoxon dan Mann-Whitney experiment. Result : The result of the research indicated significant diferences in score CPIS before and after oral hygiene using chlorhexidine (p value = 0,005) and using combination between natrium bicarbonate and listerine® with (p value = 0,036). There was no diference significant between bolt groups with (p value = 0,57). Conclusion : Chlorhexidine and the combination of sodium bicarbonate and listerine® can be used as antiseptic moutwash in the prevention of VAP.