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This study aims to measure the effectiveness of project-based learning models in instilling the entrepreneurial character and training students science process skills. This research was conducted using a pre-experimental method of one-shot case study design. This research was conducted on 20 students of Biology Education Study Program Faculty of Teacher Training and Education, University of Muhammadiyah Surabaya, Even Semester Academic Year 2018/2019. Research Data includes: (1) entrepreneurial character (KWU), and (2) science process skills (KPS). Data was collected from Several sources, Including: (1) lecture and project implementation processes, (2) scientific papers (project reports), (3) Project products (food, videos, posters), (4) the presentation of project results. Data were collected using the method of observation and Analyzed descriptively. the research of data is Categorized into four groups. The results of this study Obtained Data: (1) KWU value of students is 3:34, included in the category of "cultural" (M), and (2) Student PPP value is 2.79, included in the category of "high". The Conclusions from the results of this study indicate that the project-based learning models is very effective in instilling the entrepreneurial character and training of science process skills in biology education students.