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Objective: The research aims to determine the effectiviness of pegagan leave extrac5 on day 3 and 6 on the ability to suppress inflammation in Sprague Dawley female rats induced with S. aureus and to analyze the difference of IL-10 between group that was given antibiotics, pegagan leave extract and combination of both on day 3 and 6 after induction. Methods: This research laboratory experiment with Post-test Onl Control Group Design. The rats were divided into four groups, i.e. treatment group with Na CMC 0,5%, group with antibiotic cefadroxil 45 mg/kgBB, group with pegagan leave extract 100mg/kgBB, and group with antibiotic cefadroxil 45 mg/kgBB + pegagan leave extract 100mg/kgBB. All groups consisted of 5 rats induced with s.aureus 0,2 ml x 108 CFU (1X24 hours) and each group was given twice treatment per day (12 hours) for 5 days induction. Result: IL-10 level was measured on day 3 and 6 S. aureus with ELISA method. The results of measurement were compared between treatment groups and control group. The data were processed and analysed using One Way Anova test and T Test. The results showed that there was an increase in IL-10 level from day 3 to day 6 off all groups. However, there was no difference on IL-10 level between groups. Conclusion: pegagan leave extract is less effective to increase IL-10 level of mastitis rats.