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Formaldehyde were well known as a preservative liquid for long time ago. Especially for medical student, formaldehyde have an important part in helping them to learn of human anatomy. The use of formaldehyde it self as an embalming fluid also causes several symtoms in students. In order to decrease several negatives symptoms of formaldehyde in cadaver, Departement of Forensic Medicine and Medicolegal Faculty of Medicine Universitas Brawijaya-Saiful Anwar General Hospital has long developed an embalming mixture especially to preserve the structure of muscles and skin without no strong odor of formaldehyde, poignant and no hard consistency of cadaver. The mixture contain of formaldehyde, glycerin, alcohol, water and a few of parfume. For teaching purposes, no hard consistency and no strong odor of cadaver became most important to make the similar condition to the patient or fresh cadaver. This study use the quasi experimental design with randomized group post test only design. Used 40 rats and divided into 8 groups, 4 groups consist of positive control, treatment 1, 2 and 3 with 11.3%, 6.72% and 3.7% formaldehyde also combine with submersion, 4 other groups consists of 4 groups before without submersion. Both the embalming liquid which injected transcardial to the remains and submerged were same solution. A week after the injection and or submersion, necropsy were performed and kept for one month. Examination of the discoloration, odor production and muscle and skin structure performed. The non parametric statistic resulted significant difference between treatment 1 and 2 with submersion to treatment 1 and 2 without submersion in muscular color, odor and structure. Significant difference also showed for muscular color between treatment 1,2 and positive control. Thus can be concluded that mixture formula of Departement of Forensic Medicine and Medicolegal Faculty of Medicine Universitas Brawijaya-Saiful Anwar General Hospital could preserve cadaver muscle color and preserve structure of histological appearance of muscle and skin.