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The purpose of this study was to obtain a new strain of cellulolytic bacteria that could degrade lignocellulose waste as one of the abundant biomass into simple sugars such as glucose. Soil samples were taken from several locations in Bali and isolated using carboxymethyl cellulose (CMC)-enriched media. Screening was carried out with Congo red-polysaccharide interactions test, degradation of filter paper test and cellulase activity test. One hundred and twenty-three isolates of cellulose-degrading bacteria were isolated, able to grow and utilize cellulose as a carbon source. However, only 45 isolates produced clear zones with the Congo red test and the cellulolytic index ranging from 1.43 to 7.76 mm. Three isolates from the seven potential isolates that have the largest clear zone were proceed to the next stage of testing. The Isolates of B2S8, G14 and G22 have the highest activity of CMCase and FPase. B2S8 bacterial isolate has the highest ability to degrade cellulose on Whatman paper no. 1 was 9.82%, followed by G14, and G22 isolates which were 7.03%, and 2.94% respectively. Those with the highest CMCase activity were isolates B2S8 followed by G22 and G14, namely: 0.075, 0,074, 0.072 IU/mL respectively. While the highest exoglucanase activity was G1-4 isolate (0.112 IU/mL), followed by B2S8 and G-22, namely: 0.072 IU/mL and 0.072 IU/mL, respectively.