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Wilt disease caused by Fusarium oxysporum is one of the major diseases that cause yield loss on shallot plants. An environmentally save control method can be carried out by using biological agents. The antagonistic microorganism that has the potency to control wilt disease is Trichoderma harzianum. A carrier will be needed in a formulation of this antagonistic fungus. The formulation can also be enriched with plant micronutrient. Carbon was proven useful as a carrier of some antagonistic bacteria. Addition of silica nano particles (silica NPs.) in the formulation did not reduce the vialbility of the same antagonistic bacteria. An experiment was carried out to determine the ability of T. harzianum in the formulation with carbon fiber and silica NPs. to suppress the in-vitro growth of F. oxysporum. The experiment was arranged in the completely randomized design with 5 treatments and 5 replications. The treatments were challenging F. oxysporum by T. harzianum in different formulation on potato dextrose agar. The formulation consisted of T. harzianum + 0.5% silica NPs., T. harzianum + 1% silica NPs., T. harzianum + 0.5% silica NPs. + 5% carbon fiber, T. harzianum + 1% silica NPs. + 5% carbon fiber, and T. harzianum only. The results showed that each treatment with T. harzianum in the formulation of carbon fiber + various concentrations of silica NPs. was able to suppress the in vitro growth of F. oxysporum.