NON-INVASIVE DNA EXPLORATION METHODS IN DENTAL IMPLANT PATIENTS Pratiwi Soesilawati (a*), Mega Moeharyono Puteri (a) , Andra Rizqiawan (a)
*Oral Biology Department, Faculty of Dental Medicine, Universitas Airlangga Pedodontic Department, Faculty of Dental Medicine, Universitas Airlangga Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Universitas Airlangga
Abstract
Dental implants have become a standard therapy to replace tooth loss. The healing process around implants is a complex process to explain whether the host can receive implant material and gain a perfect recovery process. In some cases these implants are unsuccessful because the infection or lack of oseointegration. This is called "early implant failure". Some things contribute to this situation such as surgical trauma, local and systemic factors, and genetic polymorphism factors. Increased levels of MMP-1 have been shown to occur in the sulcus fluid around implants that caused oseointegrated failure and periodontitis. The increase in MMP-1 titers is related to the degradation of collagen which has the most role as an oseointegrated molecular marker. The aim of this study was to develop a non-invasive method for genetic molecular diagnosis on dental implant patients through blood sampling at dental implant sockets. Method : the total 7 subjects, consisted of 4 men with an average age of 46.7 years and 3 women with an average age of 48.3 years. Genomic DNA examination was carried out through DNA extraction using the phenol-chloroform method. DNA amplification was carried out at the MMP-1 locus at 269 bp. The results of DNA isolation were examined for DNA purity and concentration, then used as a reference for determining the amount of DNA in the amplification of the MMP-1 locus by PCR usingMMP-1 primers. Electrophoresis was carried out using 3% agarose gel stained with EtBr. The electrophoresis results of all samples showed DNA band at 269 bp. This proves that DNA exploration in the dental implant socket is a non-invasive method, safe for patients and can be used in genomic analysis methods
Keywords: DNA exploration, dental implant, MMP-1, patiens
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