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Myrmecodia or known as ant nest plant is a phytomedicine plant that traditionally used as a health supplement because of its phytochemical1. The phytochemical content such as flavonoid, phenolic, alkaloid, and triterpenoid2 might be a potential bioactive agent for synthesizing nanoparticles. Synthesizing nanoparticle using biological procedure has more advantages such as affordable, cost effective, and free of hazardous components on their surface which is safe to be used for medical purpose. Several studies investigating green synthesize different types of nanoparticles have been carried out by using leaves of the plant such as Garcinia imberti3 and Tinospora cordifolia 4. However, far too little attention has been paid in the ethanolic extract of Myrmecodia sp bulb as capping agent for biosynthesize silver nanoparticle (AgNPs). Thus, the aims of this present work was to characterize the AgNPs, synthesize using ethanolic extract of Myrmecodia sp bulb. Characterization of AgNPs was performed using X-ray powder diffraction (XRD), transmission electron microscope (TEM), Scanning electron microscope (SEM), and Fourier transforming infrared spectroscopy (FTIR) to identify possible biomolecules responsible in bioreduction of silver ions. The TEM imaging showed that the average size of resulted AgNPs was approximately 43 nm and has a crystalline spherical form as defined in SEM image. Meanwhile, the XRD analyses also revealed the average size of resulting AgNPs around 43 nm. Further, FTIR analysis green synthesized of AgNPs indicated a strong band at 2167.99 cm−1. In addition, the bands around 3873.06 cm−1, 1612.49 cm−1, and 2337.72 cm−1 indicating –OH, C–C and C=O stretching of hydroxyl groups, alkenes and alkanes, respectively. Present research provides is beneficial in the green biosynthesis of AgNPs using Myrmecodia sp bulb ethanolic extracts, having their AgNPs physical-chemical properties which might useful as antibacteria and other medical purposes.