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Awareness of tourism destination sustainability is important to protect the destination from damage and over exploitation. Studies on tourism destination sustainability are still in the early stages. Scholars have just developed the indicators and instruments that will be used to measure the destination sustainability. Factors affecting tourism destination sustainability, especially the culture-based destination, have not been explored systematically using valid and comprehensive instruments. We present regression analysis of potential antecedents of culture-based tourism destination of 5 cultural destinations in Central Java involving 200 local people. The results suggest that resources and innovations have the strongest effect to the sustainability of culture-based tourism destinations. They are complemented with tour guides- performance. This indicates the importance of tour guides- training and orientation in building tourists- awareness. Resources provision and innovation are also crucial strategies in maintaining culture-based tourist destination sustainability.