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Water is a basic need for human life and other living things that must be maintained so as not to degrade water quality. Rivers as the biggest water supplier are often unavoidable from pollution problems. Karang Mumus River is one example of a river that has pollution. River pollution is caused by the many activities of the community around the river so that increasing pollutants entering the river body. Communities in the area have limited access to clean water so they have no choice but to use river water for bathing, washing clothes and washing kitchen utensils. This is very worrying because it can cause health impacts on the community. The method in this research are observation, interview, and measurement of water samples. Water Sampling was taken for 8 points. The determination of sample points is based on potential sources of pollutants, which is densely populated areas. The results of measurement of Escherichia Coli in all water samples showed that the content of Escherichia Coli was the lowest <30 CFU / 100 ml and the highest was 2400 CFU / 100 ml. The content of Escherichia Coli in all water samples has exceeded the water quality standard for hygiene sanitation.