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Extraction of bioactive components in rice bran is influenced by several factors, one of them is the type of solvent used. The rice bran var. IR-64 samples were obtained from Sukoharjo, Central Java, Indonesia. The aims of this research are to know the difference of antioxidant activity by defatted and non defatted treatment and to know the optimum temperature extraction of bioactive component in rice bran. The first step treatment is defatted rice bran extraction with using N-hexane and non defatted using ethanol 96% solvent. The advance step is non defatted treat used the temperature variation of ethanol 96% solvent. The temperature treatment used were 500C and 700C. The rice bran extract obtained will be analyzed yield, total phenol content using the folin ciocalteu reagent and antioxidant activity test carried out by the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The results of the first stage research in non-defatted and defatted treatments showed that the antioxidant levels of rice bran extract did not significantly. Then the research continued to advanced treatment with variation of the temperature of ethanol 96% in the non defatted treatment. The results show that temperature treatment can increase significantly antioxidant activity in rice bran extract. The yield obtained in the non-defatted treatment was 17.27% with antioxidant activity of 62.52% and phenol content of 15.53 mg GAE/g. While the yield on the defatted treatment showed a result of 0.48% with an antioxidant activity of 69.97% and a phenol level of 5.334 mg GAE/g. Non-defatted treatments with temperature variations of 500C and 700C with 96% ethanol solvent obtained yields respectively 9,901 % and 7,934 % with antioxidant activity 82,086 % and 77,515 % and phenol content of 9,407 mg GAE/g and 5,938 mg GAE/g. The temperature in the extraction process affects the antioxidant activity of rice bran extract