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This research aims to determine the effect of varieties on the growth and yield of chili plants on the Andisol soil. This research was carried out at Lon Baroh, Lembah Seulawah District Aceh Besar, greenhouse and laboratory of plant physiology, Faculty of Agriculture of Syiah Kuala University, Darussalam, Banda Aceh from November to March 2018. The analysis of data used in this study was Randomized Block Design – non Factorial with 3 replications. There are 2 series of this research, Serie A and Series B. Serie A is used to collect data on growth and yield of chili plants and Serie B is used to collect data on mycorrhizal infection. The factors observed in this research were varieties. Varieties used are Lado F1 and Perintis.The result of this research showed varieties of chili plants highly significant take effect on plant height 15, 30 and 45 DAP, stem diameter 30 and 40 DAP, productive branch numbers, plant fresh weight, plant dry weight, fruit weight, fruit length, fruit number and yield potential and significantly affect on stem diameter 45 DAP, root fresh weight, and root dry weight. The best growth and yield of chili plants is found in Lado F1 varieties.