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The aim of this study was to know the incidence of repeat breeding in dairy cows and to know the effectiveness of Heatsynch protocol on dairy repeat breeding cows. The study was conducted in two stages. The first stage, all dairy cows were recorded and clinically examined through palpation per rectum and for their reproductive statuses. Parameter measured in the study was the incidence of repeat breeding. The second stage of the study was that the repeat breeder cows subjected to Heatsynch protocol for estrus induction. The protocol was GnRH injection on day-0, followed by PGF2α, estradiol injections on day-7 and day-8, respectively, and inseminated artificially on day-9. The results of the study indicated that the incidence of repeat breeding was 41.6%. The Heatsynch protocol was successfully increase the number of pregnant repeat breeder cows; 75%, higher compared to untreated repeat breeder cows; 20%. It can be concluded that high incidence of repeat breeding in dairy cows. Heatsynch protocol can be used to treat repeat breeder cows and improve the reproductive efficiency.