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Koi fish is one of high economic value fish because of its beautiful body shape and colour. Its colour is influenced by the protein and carotenoid-rich feed. The sources of protein and carotenoid used as supplement were chitosan and chlorella. The purpose of this study to evaluate the effect of protein and carotenoid-rich feed using chitosan, Nanochitosan emulsion and chlorella on growth performance, water quality and protein retention of Koi Fish. Experiment was conducted by preparing the nanochitosan emulsion and formulated as Koi fish feed. The growth performance and protein retention of Koi fish and water quality were evaluate using ANAVA. In the experiment, Koi fish (mean initial wet weight 1.49 g) were fed with three diets (control, chitosan and nanochitosan emulsion) in triplicate for 60 days. The result shows that growth performance of Koi fish fed nanochitosan emulsion diet was higher (P<0.05) than other diets. Koi fish in chitosan diet group was higher (P<0.05) than of Koi in control but no statistical difference was found in Koi in nanochitosan emulsion diet groups with protein retention 57.03 ± 1.76 and the water quality showed Ph 7.32, DO = 6.23, NH3 = 0.03, NO2 = 0.0075 and NO3 = 0.03 mg/L.