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Background: Candidiasis si a fungal infection caused by Candida albicans. Synthetic drugs are often used in candidiasis treatment. Using synthetic drugs for a long period can leads to antifungal resistant phenomenon. Alternatif medicines from plants can be used as alternative to evercome this problem. Srikaya Leaves (Annona squamosa) are one of the sources of natural ingredients that have been shown to have various effects, including antifungals effect. Method: This study was aimed to determine the antifungal effects of srikaya leaf extract in inhibiting the growth of Candida in vitro. Srikaya leaf extract was obtained by maceration. After that, the extract was diluted into various concentrations (10% b/v, 20% b/v, 40% b/v, 80% b/v, and 100% b/v) with DMSO. Agar wells difusion method was chosen for testing the extracts antifungal effect. Tests were carried out 5 replications for each concentration. Results: Each concentration used in this study did not show any inhibition zone in the growth of Candida albicans in vitro, the same was found in negative controls. While positive control with Nystatin 20 mg showed average inhibition zone of 19 mm. Conclusion: Srikaya leaf extract has not been able to inhibit the growth of C. albicans in vitro. This can be caused by various factors, including the method of extraction and selection of fungal isolates. It is recommended to use a better extraction method and pure isolates of Candida albicans for further study.