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Nebulizers are commonly used device for generating aerosols. In medical fields, the medical-grade nebulizers are used as drug delivery via inhalation. This involves the aerosolization of drugs in solution or suspension forms. In aerosol research field, commonly laboratory-grade nebulizers have a slightly different design to those of medical nebulizers as they optimize the aerosol generation stability and production rate with less deposition. This paper will discuss the utilization of medical grade nebulizer to generate aerosol from various solutions and suspension, which are usually used in aerosol research, i.e. NaCl, gliserin, dioctyl sebacate (DOS), and polystyrene latex (PSL). The size distribution of the aerosol was measured using scanning mobility particle sizer (SMPS). We found that the concentration of NaCl and gliserin solutions directly affected the geometrical mean diameter (GMD) and geometrical standard deviation (GSD) of the generated aerosol. The test using PSL particles depicted that the medical nebulizer had a relatively high aerosol deposition, which increased the suspension concentration over time.