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MORPHOLOGICAL CHARACTERIZATION AND SUGAR PRODUCTION OF TRANSGENIC SUGARCANE RESISTANCE AGAINST MOSAIC VIRUS
Choirul Ainiyati1,Bambang Sugiharto1, Sudiarso2, Soemarno2*

1 Faculty of Agriculture, Brawijaya University, Jalan Veteran, Malang 65145, Indonesia
1Laboratory of Molecular Biology and Biotechnology, Center for Development of Advanced Sciences and Technology (CDAST), University of Jember, Jln. Kalimantan 37, Jember 68121, Indonesia, 2 Faculty of Agriculture, Brawijaya University, Jalan Veteran, Malang 65145, Indonesia


Abstract

Background: SCMV (sugarcane mosaic virus) is one of the important pathogens in sugarcane, it-s can reduce sugar production around 30-50% (Dewanti et al., 2015). Genetic engineering is one way to develop virus-resistant plants, and the SCMV-resistant sugarcane has been obtained using PDR (Pathogen Derived Resistance) techniques through genetic transformation (Apriasti et al., 2018). It has been proven that the transgenic sugarcane shows resistance to SCMV infection, but growth performance and sugar production has never been characterized. This study aims to characterize the morphological and sugar production of transgenic sugarcane and comparing to non-transgenic sugarcane. Aims : To characterize of growth performance and sugar content of transgenic sugarcane resistance against mosaic virus. Materials and Methods: This study uses bululawang as a non-transgenic sugarcane, transgenic sugarcane (SCMV-resistant sugarcane by overexpression of the gene encoding the coat protein (CP) of SCMV), and Variety of PS.864 and Ps.881 (sugarcane infected by ScMV as a source of disease). The Sugarcane plants were grown for nine months, naturally-infection of mosaic virus by grown of infected plants between transgenic plant plot. The sugarcane leaves at the age of 3 months were collected for analysis of resistance to mosaic virus using RT-PCR and Western Blot. Morphological traits of transgenic sugarcane were observed for three time at the age three, six, and nine months after planting, whereas brix on stem was measure only at the age nine months after planting Results: The morphological traits include the number of plants, plant height, stem diameter and length of internodes did not show significant difference between sugarcane transgenic compared by Non-transgenic. However, the brix in transgenic sugarcane exhibited enhance compared to non-transgenic sugarcane. Conclusion: The SCMV-resistant sugarcane have not difference of the morphological traits compared to non transgenic sugarcane, whereas the brix on stem enhanced in transgenic sugarcane. References 1. Apriasti R., Widyaningrum, S., Hidayati, W.N., Sawitri, W.D., Darsono, N., Hase, T., Sugiharto, B. 2018. Full Sequence of TheCoatProtein Gene isRequiredforTheInduction of Pathogen-DerivedResistanceAgainstSugarcaneMosaic Virus in TransgenicSugarcane, Molecular BiologyReport. Vol 45:2749-2758. 2. Dewanti, P., Widuri, L.I., Ainiyati,C., Okviandari, P., Maisaro, Sugiharto, B. 2016. Elimination of SCMV (SugarcaneMozaik Virus) and Rapid Propagation of Virus-free Sugarcane (SaccharumofficinarumL) UsingSomaticEmbryogenesis, ProcediaChemistry, Vol 18: 96-102

Keywords: PDR (Pathogen Derived Resistance), Sugarcane Mosaic Virus, SCMV-resistant sugarcane.

Topic: Environmentally Sustainable Agriculture

Link: https://ifory.id/abstract/cx3j26KpFMH4

Conference: International Conference on Bioenergy and Environmentally Sustainable Agriculture Technology (ICONBEAT 2019)

Plain Format | Corresponding Author (choirul ainiyati)

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