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The research reported herein is to reveal the effect of in ovo injection (IOI) of L-gln into the eggs of Indonesian native chicken on hatching weight and subsequent weights at 4 and 8 weeks of age. The research was arranged as a factorial experiment 2 x 5 based on a completely randomized design with unequal replication; the first factor was L-gln (L) in isotonic NaCl, with 5 levels: P0 – negative control (without injection), P1 – positive control (isotonic sterilized saline), P2, P3 and P4 – 0.5, 1.0, and 1.5% respectively, and the second was sex (S), with 2 levels (male and female). As much as 97 newly hatched chicks were selected, weighed, tagged the wing, and used in this study. Data were analyzed by variance analysis. The results indicated that from hatching day up to 3 weeks of ages, the weights of newly hatched chicken either with the injection of different levels of L-gln or between the two sexes were not significantly different. There was a strong relationship between egg weight and day old weight of newly hatched chicks, but no significant relationship at further ages. Differences between the two sexes and different levels of L-gln showed a significant effect at 4 weeks of age and continued at 8 weeks. The weights of all IOI chicks were heavier compared to that of the negative control chicks.. The weight of male chicks was heavier compared to that of the female, and there was no significant effect of increasing level of IOI L-gln. The weight of the female chicks increased gradually with increasing level of L-gln. In conclusion, in ovo injection of L-gln positively improved the productivity of Indonesian native chicken in both sexes.