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Red dragon fruit (Hylocereus polyrhizus) is one of cactus plant group containing iron, vitamin B1, Vitamin B2, vitamin B3, Vitamin C, and others. Vitamin C is a water-soluble vitamin can be antioxidants. People commonly store red dragon fruit because its considerable size and does not consume a single meal, however length storage can decrease nutritional value of the fruits because vitamin C is easily oxidized . The aim of this study was to determine effects of length storage toward levels of vitamin C in red dragon fruit during 1 until 3 days. The study was experimental design through cross sectional approach. 3 samples were taken by random sampling were purchased at Pasar Buah Kota Jambi. Vitamin C levels used Iodimetry method were conducted at the Chemistry Laboratory of the Academy of Health of Jambi Province. The results showed that average vitamin C level in red dragon fruit in 1 day storage was 12,1405 mg, 2 days storage was 11,0369 mg and 3 days storage was 6,9860 mg. Statistical test used the ANOVA test concluded that there was an effect of vitamin C levels on dragon fruit stored for 1 day, 2 days and 3 days.