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Fluor albus is a symptom very often experienced by most women. Fluor albus is classifie into two types, namely, physiological and pathological fluor albus. The pathological fluor albus is caused by infection or inflammation which can lead to many problems in the reproductive health of teenagers. The purpose of this study was to analyze the effectiveness of health education on the knowledge, attitude and motivation about vaginal hygiene and prevention of fluor albus in An-Najah Aliyah School female students. Research design was pre-experimental pretest and posttest one group design. The population of this study were 130 female students of An-Najah Aliyah School, from which a sample size of 64 female students were selected. Sampling was conducted with proportional stratified random sampling technique. The research data were analyzed with the Wilcoxon test. The results showed that there were significant differences in the knowledge, attitude and motivation of the female students between before and after being given health education about vaginal hygiene and prevention of fluor albus (p value < 0.05). The conclusion is that health education is effective in increasing the knowledge, attitude and motivation about vaginal hygiene and preventing fluor albus of female students in Aliyah An-Najah School.