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Background: Hospitalization can affect the child-s behavior as a reaction to the experience of hospitalization. These reactions are individual and depends on the stages of child-s development, previous experience of illness, the available support systems, and the coping abilities they have. Purpose: The aimed of this study was to determine the relationship between the role of parents toward the impact of hospitalization on children in RSUD Kota Kendari. Methods: This research design was analytic observational with cross sectional approach. Samples in this study were pre school and school ages children who were being treated as the patient, where the respondents were parents of patients with 32 samples taken by total sampling technique based on inclusion criteria. Data analysis was using statistical chi-square test (α = 0.05) with significance level 95%. Results: The results showed that the role of parents in the good category were 26 people (81.2%) and the impact of hospitalization on children with positive categories were 25 people (78.1%). The results of the analysis obtained the value of fisher exact test showed the relationship of parents role towards the impact of hospitalization on children, with a value of p = 0.012 <α = 0.05. Conclusion: There is significant relationship between the role of parents towards the impact of hospitalization on the children in RSUD Kota Kendari, where the more better of parent-s role it will be getting a positive impact of hospitalization experience to the children.