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The research aims to dtermine pollutant content in oil industry wastewater by using probiotic bacteria in reducing the content of pollutant in the palm oil industry. This study was conducted in Laboratory of Microbiology, Faculty of Medicine , Hasanuddin University, Makassar and Health Laboratory Center (BBLK). The sample was determined using random sampling method. The data were analysed using Variance (ANOVA) method. The results of the research indicate that there are two types of bacterial isolates, , namely isolate A was Lactobacillus bulgharicus and isolate B was Lactobacillus plantarum. Probiotic bacteria isolated from cow-s milk can reduce BOD content from 250.25 mg /L to 95.56 mg /L. COD value decreases from 530.25 mg /L to 245.81 mg /L. TSS value decreases from 420 mg /L to 232 mg /L. The initial fat content decreases from 30.25 mg /L to 21.65 mg /L. The initial measurement of value decreases from 7.5 to 6. Biodegradation process of the palm oil industry wastewater with the addition of the probiotic bacterial isolates can reduce organic content and pollutant content of the liquid waste of palm oil industry. The most effective rate of waste degradation is bacterial isolate Lactobacillus plantarum with McFarland 1.5.