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Background: Anther culture is a tissue culture technique that uses pollen to produce plants. Anther culture shortens the acquisition time of pure lines from 6-8 generations of plants to 1-2 generations [1]. The success of tissue culture can be seen by the high regeneration power of callus that is formed. Factors that can affect one of them is the difference in nutrition and growth regulators given. The addition of growth regulators in the form of IAA and Kinetin is expected to be able to handle the problems that occur in the regeneration process of anther callus. This research uses combination media A1: 0 ppm, A2: 1 ppm, and A3: 2 ppm. The second factor is the concentration of Kinetin with 4 levels B1: 1 ppm, B2: 1.5 ppm, B3: 2 ppm, and B4: 2.5 ppm. Observation variables include 1) Green Spot, 2) Brown Callus, 3) Plantlet, 4) Root. The results showed that the treatment of 2 ppm IAA + 2.5 ppm Kinetin gave a good response of anther callus regeneration, 12.5% plantlets formed and appeared when the callus was 30 days marked by budding, 62.5 % green spot formed; 0% browncallus, 12.5% plantlet; and 50% rooted callus. Aims: To obtained the right concentration of IAA and Kinetin for the regeneration of black rice plant anther callus in vitro. Materials and Methods: The materials used in this study were black rice anther callus from previous studies; 0.4gr / 100mL N6; 0.3gr / 100mL Phytagel; 3% sucrose; 0.05gr / 100mL glutamine; IAA and Kinetin, 70% alcohol; NaOH; HCl; and aquadest. Callus subculture the calli of 1-2 mm diameter with criteria callus friable to N6 regeneration media which includes a combination of IAA (0; 1; 2) ppm, Kinetin (1; 1.5; 2; 2,5 ) ppm. The regeneration callus that had been transferred to the regeneration media was incubated in a sterile room in a room without light for 7 days for a total of 8 calluses per bottle. The regeneration callus was then placed in a no-light condition (dark room) for 7 days. After that, transferred to a room with light for 16 h at 28±2ºC and light intensity 1500-1600 lux until a plantlet appears. Subcultures need to be done further because at the age of ± 14-21 days the callus that has been subcultured previously shows a change in size and if it takes too long the callus will turn brown and the regeneration power of the callus decreases. Results: The anther callus used in this study was an induction callus that was produced from a previous study, Bantul black rice variety using MS induction media; 0.5 ppm 2,4-D; 2.5 ppm NAA; and 0.5 ppm Kinetin. Callus regeneration required higher cytokinin concentrations compared to auxin [1]. Each increase in the concentration of cytokinins is able to show a tendency for the appearance of callus, shoots, and leaves more quickly, and plays an important role in the regeneration of the callus used [2]. Regeneration media 2 ppm IAA + 2.5 ppm Kinetin showed the highest number of green spots in the regeneration callus compared to other treatments 62.5%. Treatment