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Rhizobium is one of the important microorganisms in free nitrogen fixation. The study aims to determine the use of Rhizobium spp as substitute for chemical fertilizer Nitrogen on soybean cultivation. The study was conducted in the village of Sukorejo, Bangsalsari Subdistrict, Jember Regency from May to August 2019. The experiment design was a randomized complete block with four replications, using Rhizobium isolate in granule and powder media with spraying volume 600cc/kg granules and 400cc/kg of powder. The composition of the granules and powder media consists of edamame soybean waste, zeolite, phosphatealam, kaptan and tapioca. Rhizobium isolates were obtained from Protection Laboratory of Politeknik Negeri Jember. The treatment dosage consisted of 3 g; 4 g; 5g and 6 g/plant, while the control was Phonska fertilizer 1,5 g/plant (180 kg/ha equivalent). Research data were analyzed by Anova and Dunnet test. The results showed that 1) The use of rhizobium granules and powder as much as 4.0 g / plant was not significantly different in the formation of root nodules compared with 1.5 g / plant fertilization at 56 DAP and 70 DAP; 2) Number of root nodules 56 DAP with 4 g/plant was 23.66 (granules), 23.25 (powder) and 20.60 (control); 3) The number of 70 DAP root nodules with granular fertilizer and powder of 4 g/plant respectively was 24.20; 23.96; and control 22.90; 4) The use of Rhizobium in granule media 6 g/plant for the number of pods did not show a significant difference compared to controls (180 kg Phonska/ha); 5) The use of granules of 4 g - 6 g/plant produced significantly different seed weights compared to controls, namely 13.67 g and 13.95 g. The use of Rhizobium isolates 600cc/kg granules and 400cc/kg of powder applied to 4-6 g/plant can be used to replace the use of chemical fertilizer Nitrogen Phonska as much as 180 kg/ha in soybean cultivation.