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Introduction: Dengue Haemorrhagic Fever is a public health problem in Indonesia. The nation capital Jakarta has the most cases compared to other provinces. Rawasari District was declared an endemic area for Dengue Haemorrhagic Fever (DHF). Eradication of DHF centred on vector (Ae. aegypti) control. Organophosphate is the insecticide of choice for over 25 years of vector control effort. Insect resistance to organophosphate is marked by an increase of non-specific esterase enzyme activity that can be detected using microplate assay. Method: This study collects Ae.aegypti and larvae randomly from houses in the research area and conduct alfa esterase activity assay using microplate reader. Briefly, larvae homogenate placed inside ice cooled microplate wells. The prepared reagent used to assessed esterase activity from each homogenate and evaluated using spectrophotometer at 450 nm. Sampling locations were determined geographically using Google Earth. Results: Organophosphate resistance pattern from Rawasari district area shows a 22.4% high resistance, 41.1% moderate resistance and 36.4% larvae still sensitive to organophosphate. Discussion: Resistance pattern shows in this study was similar to other study conducted in the greater Jakarta area. However, a large proportion of the vector has moderate resistance, which warrants a vector resistance surveillance program. Vector control program conducted at Rawasari district can still use organophosphate to eliminate DHF vector.