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This research intends to analyze the linkage of migratory culture and entrepreneurship through information acquisition. The research population are migrant Madura entrepreneurs in East Java, Indonesia, with a sampling technique using a proportional random sampling technique at three locations: Surabaya City, Malang City and Jember Regency. The number of respondent are 170 Madura migrant respondents. The consideration is that there are relatively many Madura migrants in those cities. This research was carried out by designing questionnaires first and then validity dan reability test of the research instrument. Further, the questionnaire was broadcasted to Madura migrant entreprenuers who were randomly selected. The primary data was analyzed by structural equation model with the Partial Least Square (PLS) data processing program. The research result demonstrate that migratory culture significantly effect entrepreneurship and information acquisition mediates the effect of migratory culture toward entrepreneurship. It means that information acquisition plays an important role to encourage entrepreneurship of madura migrants.