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Abstract Objective: This study aimed to determine the effectiveness of C. Asiatica leaf extract to TNF-α levels in female Sprague Dawley rats induced by S. aureus. Methods: There were four treatment groups (each group consisted of five rats). Group I was given 0.5% Na CMC, group II was given Cefadroxil 45 mg/kg WB, Group III C. asiatica leaf extract 100 mg/kg BW, and group IV combination Cefadroxil and Centella leaf extract. Each group was given treatment twice per 12 hours a day for 5 days. Results: TNF-α level between groups did not have a significant on day 3 and differed significantly after day 6. On day 3, the control group had a higher TNF-α level of 25.13 pg/ml compared to the group given antibiotics and C. asiatica. While when compared to the group given only C. asiática leaf extract, the control group was more height of 17.1 pg /ml. On the 6th day, this condition was changed, the biggest difference found in the group given C. asiatica, in the control group had higher levels of TNF-α 72.34 pg/ml compared to the group receiving C. asiática. Then, control group is higher than 66.46 pg/ml compared to those given antibiotics and C. asiatica. Conclusion: C. Asiatica leaf extract is effective in reducing TNF both given alone and given along with antibiotics. It is potential to be explored into alternative and complementary treatments in mastitis cases with human trials