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Abstract Objective: To determine the ability of brown algae (Sargassum sp) extract 15% in dissolving canal smear layer. Conservative Dentistry Department, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia Results: Root canals that were irrigated using brown algae (Sargassum sp) extract 15% showed a significant decrease of smear layer compared to other groups (p<0.05) either in 1/3 middle portion or 1/3 apical portion. Conclusion: Brown algae (Sargassum sp) extract 15% was more effective to clean smear layer on root canal wall compared to NaOCl 2.5% but did not show a significant difference compared to EDTA 17%.